Medium for SSF as much as 20 mL consists of delignificated bagasse samples (1 g); nutrients (NH4)2HPO4 (3.44 mL), MgSO4.7H2O (0.17 mL), yeast extract (6.88 mL); citrate buffer (pH 5.0); cellulose enzymes (20 fpu) and 25 % (v · v–1) of yeast Saccharomyces cerevisiae. SSF was conducted on ambient temperature and pressure with three variables incubation period of 3 d, 5 d and 7 d.