The extractions for both D. melanogaster heads and thoraxabdomens were run in triplicate on LC/QTOF-MS, with 3 total ion
chromatograms (TICs) for each determination. Extractions from
heads of 5 separate D. melanogaster cultures (5 determinations),
extractions from thorax–abdomens of 4 separate cultures (4 determinations), and [1-13C]-palmitic acid incubation extractions from
heads (3 determinations) were analyzed