Frozen samples were thawed to room temperature (28 C) and 10 g of each sample was homogenized with 90 mL buffered peptone and was properly blended. 1 mL dilutions were inoculated on to TPC petrifilm (PetrifilmR 3M) to obtain Total Plate Count and incubated at30Cfor 48h. Furthermore,1 ml dilutions were inoculated on to E coli petrifilm (3M™ Petrifilm E coli/Coliform count plates) and Baired Parker medium (Oxoid, UK) to obtain E. coli and S. aureus count respectively at 37 C for 24 h.