Ultra-high speed analyses of amino acids were undertaken using active flow technology chromatography columns with MS detection. The AFT columns employed here had internal diameters of 2.1 mm, and these columns were operated at 4.5 mL/min. Subsequently, separations of the amino acids were feasible in less than 15 s, with injection to injection precision generally less than 5%, dependent on the standard concentration. Because these AFT columns enabled very high flow rates to be employed with MS detection, high sample through-put was achievable that effectively emulated direct injection protocols.