The EO has shown antibacterial activity against anaerobic Gram-negative bacteria P. nigrescens and P. gingivalis (MIC 100 μg mL−1). For other aerobic bacteria and all anaerobic bacteria tested, the EO demonstrated moderate activity and/or it was inactive. Regarding the fractions, F1 showed similar results to the EO, except for anaerobic bacteria P. nigrescens and P. gingivalis that showed a slight improvement in activity. As discussed above, F1 is predominantly composed of sesquiterpene hydrocarbon. Probably, the biological properties of the EO can be justified by the characteristics of the sesquiterpene hydrocarbon, because F1 was more active for the same bacteria as the EO.