FIG. 4. Time-dependent reaction of human LDL (2.5 mg/ml)
with glucose (200 mM). Samples were dialyzed and portions separated
into apoprotein (unit of AGE per pg of apoprotein) (A) and lipid
(unit of AGE per pug of lipid) (B) components for AGE determination
or assayed for the presence of oxidized LDL (pmol of MDA
equivalents per pug of LDL) (C). A, LDL incubated with 200 mM
glucose; A, LDL incubated with 200 mM glucose and 300 mM
aminoguanidine; o, LDL incubated alone; e, LDL incubated with
aminoguanidine. Values shown are means of duplicate determinations.