Preparation of sesame seed extract
Ground and defatted sesame seeds (250 g) were extracted with
2.5 L ethanol in an ultrasonic bath for 2 h at room temperature. The
mixture was centrifuged at 4000 rpm for 20 min (SV11RH, FIRLABO,
IATRIN, Thessaloniki, Greece) and the supernatant was evaporated
to dryness under vacuum at 40
C using a rotary evaporator. The
residue was weighed, dissolved in an appropriate volume of
ethanol and stored under refrigeration.