Seeds of the non-heading Chinese cabbage (B. rapa subsp. chinensis) (land race dabadaba, van Averbeke et al., 2007) and black nightshade (S. retroflexum) were obtained from Mr. Mabulannga, a smallholder farmer at Dzindi Irrigation Scheme (Itsani village) in Limpopo Province of South Africa (23°01′45″S and 30°26′30″E).