Introduction: Mantis shrimp (Oratosquillanepa) muscle obtained from by-catch resources was used as an alternative source for mammalian collagen. Objectives: The aim of the study was to isolatethe Acid soluble collagen (ASC) and Pepsin soluble collagen (PSC) from body muscles of Mantis shrimp Oratosquillanepa. Methods: Body muscles of O. nepa the exoskeleton was removed, the muscles were cut into small pieces (0.3-0.5 cm) and stored at -4°C until used. O. nepawas extracted with 0.1M NaOH to remove non-collagenous protein for 3 days. Then, the deproteinised muscles was washed with distilled water and lyophilized for further analysis. Results: The net yield of ASC and PSC was provisionally estimated as 42% and 23% respectively in dry weight basis. The molecular masses of the ASC and PSC subunits (α1, α2) were about 105 kDa and 96 kDa. According to the FT-IR spectrum, both ASC and PSC results showed the chemical shifts of amid regions were characterized as Type I collagen. Conclusion:These finding shows the great potential of Mantis shrimp muscle collagen as a new source biomedical materials, food andnutraceutical industries.