DNA was purified using the QIAamp DNA Blood Mini Kit(Qiagen), and quantified using NanoDrop (Life Technologies) and Qubic (Life Technologies) platforms. DNA quality was further evaluated by PCR analysis using the BIOMED 2 PCR mutiplex protocol with PCR products analyzed by DNA 1000 Assay (Life Technologies) on the Agilent 2100 Bioanalyzer on-chip electrophoresis (Agilent Technologies), as previously described [16].