Ultimately, artificial mixtures of the compounds were used in a biological assay system to demonstrate the relative activity of individual compounds. Although over 80% of the volatile compounds could be identified, this seemed to be adequate to achieve an excellent reproduction of the lethal antibiotic effects of the VOCs that were being produced by the fungus. We then examined each of the five general classes of VOCs in the bioassay test and each possessed some inhibitory activity with the esters being the most active . Of these, the most active individual compound was 1-butanol, 3-methyl-, acetate