A Autoradiograph of tomato RNA probe with ³²¯P-labeled tomato fruit fructokinase cDNA. Total RNA (15 ug each lane) extracted from different organs of tomato plant as well as from different stages of fruit development (marked in days after anthesis, DAA) was separated by gel electrophoresis and transferred to a nylon membrane and probed. B. Immunoblot analysis of fructokinase. Soluble protein (5 ug each lane) extracted from different organs of tomato plant as well as from different times in fruit development were separated on a 12% SDS-PAGE, blotted to nitrocellulose and probed with antibodies raised against tomato fruit fructokinase. C. Fructokinase activity.