2.5. Coefficient of total tract apparent digestibility and N balance studies
Daily urine production was collected separately beneath the metabolism crate in a plastic container and recorded. To
avoidNvolatilisation, 20cm3 (25%) sulphuric acid (H2SO4) was added to the container daily.Aweak solution (2%) of sulphuric
acid was applied to the connecting funnel twice daily. A 50ml aliquot of urine and 100 g aliquot of faeces were retained daily
and frozen (−20 ◦C) for subsequent determination of N.