The effects of the multi-enzyme on viscosity reduction of cassava substrates were studied under conditions for VHG fermentation. Cassava roots (unpeeled) were chopped and homogenized using a blender, and the solid content adjusted to 32% (w/w) on dried weight basis with tap water. Ground cassava chips were used for preparation of slurry at 32% (w/w) solid content. Fresh cassava pulp was adjusted to 10% (w/w) solid content. The substrates were adjusted to pH 5.0 and treated with 0.05–2.5% (w/w) of the multi-enzyme at 45 °C for 2 h with 160 rpm mixing. Samples were collected after 0, 30, 60, and 120 min of enzyme treatment for viscosity analysis using a Rapid Visco Analyzer (RVA 4, Newport Scientific, Australia) at 32 °C with an agitation speed of 160 rpm for 3 min. Reactions with no-enzyme were used as controls. The experiments were done in duplicate. The target operational viscosity is set at