Materials and methods
Test organism
Theexperimentson xylanase productionwereperformed with a
strain of
A. lentulus
FJ172995 previously isolated (
Sharma, 2009
)
from the textile ef
fl
uent procured from Baddi (Himachal Pradesh,
India). The fungal isolate was maintained on slants of Potato
Dextrose Agar. Freshly revived cultures were used for all the
experiments.
Screening of A. lentulus for xylanase production
The fungal isolate
A. lentulus
was initially screened for xylanase
production on xylan-agar plates containing 1% xylan and 0.1%
Congo Red (
Bajaj and Singh, 2010
). Plates were spot inoculated with
the fungal spores and incubated at 30
C for 5 days. The diameter of
the clear zone formed around the fungal colony was measured on
daily basis.