Biofuel production using algal biomass calls a great interest in many research teams. Among the numerous species, phototrophic microalgae are certainly attractive, whose needs for growth are light, carbon dioxide and some quantity of mineral substances. These are very important factors in biofuel production. The biomass concentration in a bioreactor depends on its type. Thus, it may be up to 0.5 g/l in open reactors, and up to 5 g/l in closed systems (Vandamme et al., 2013).