we did additional extensive surveys and collections at the island of
Bangkulu in 2004.Wherever possible, we entered the water and scored
for presence or absence of P. kauderni. In addition, when possible, fish
were collected and these samples constitute the second set of samples.
This second set comprised 273 individuals collected at 11 sites (Table 1,
Fig. 2). Of those 11 sites, 3 corresponded to sites sampled for the Bernardi
and Vagelli (2004) study (sites 2, 5 and 6) and at these three sites we
collected additional fishes (no additional samples were obtained from
site 4 of Bernardi and Vagelli, 2004). We also sampled an additional 8
new sites (Table 1, Fig. 2).We collected samples with a hand net, and the
individuals were fin clipped and then returned to their habitat.We stored
fin clips in 95% ethanol at ambient temperature.