3.1. Calculation of theMolar Absorptivity Coefficient by HPLC and Spectrometric Method. Few attempts have been made to
calculate absorption coefficient for capsaicinoids.The majority of capsaicinoids exhibit absorption in the UV region of
the spectrum, between 200 and 350nm. Because these obey the Beer-Lambert law(absorbance is linearly proportional to
the concentration), absorbance measurement can be used to quantify the concentration of a pure (standard) capsaicinoid
or to estimate the total capsaicinoid concentration in a mixture or extract of capsaicinoids in a sample. Standards
of capsaicinoids (capsaicin and dihydrocapsaicin) commercially purchased were dissolved in acetonitrile and the spectrum
was scanned in order to evaluate their fine structure (Figures 1(a) and 1(b)). Using the absorbance measured at its
maximum wavelength (280 nm) and taking into consideration the dilution factor, purity degree, and molecular mass
(capsaicin 305.41 g/mol; dihydrocapsaicin 307.43 g/mol), the