Binding of GDP, GTP and GDPNP to EF-G For the experiment shown in Figure 2a, 5 M EF-G was incubated for 2 min at 37°C with [3H]-GDP (3-18 M) in 50 l. The tubes with EF-G and GDP were then put on ice.
Binding of GDP, GTP and GDPNP to EF-G For the experiment shown in Figure 2a, 5 M EF-G was incubated for 2 min at 37°C with [3H]-GDP (3-18 M) in 50 l. The tubes with EF-G and GDP were then put on ice.