Antibody incorporation in polydiacetylene (PDA) liposome was used to specifically detect Salmonella.Modifying physical properties of PDA liposome, such as size, surface charge, and packing of lipids, could affect the sensitivity and the stability of it. Therefore, the main goal of this study was to evaluate fourfactors simultaneously in PDA/antibody vesicles to detect Salmonella. Raman spectra were carried out