The egg collection and storage practices were repeated when the flock age reached 37 wk. For each trial, a total of 270 eggs per storage treatment were
incubated at 37.5°C and 56 to 57% RH, and in the last 3 d, the eggs were transferred into a hatcher with a temperature of 36.9°C and 60% RH. Beginning at 4 d and up to 21 d of incubation, 30 eggs (15 eggs/storage treatment) were opened to determine daily embryonic wet weight.