2.6.2. Ferrous ion chelating ability
The chelating ability on ferrous ion was determined by the decrease in absorbance at 562 nm of the iron (II)eferrozine complex using the protocol as described by Dinis, Madeira, and Almeida (1994). The chelating effect of the test FSE on ferrous ion was calculated using the formula as follows: Chelating effect ð%Þ¼ ðAC A AT Þ C
100
AT ¼WAhberseo,rbaAnCce o¼f theAtbessotrsbaamncpele. of the control solution;