2.2. Methods
2.2.1. O-Carboxymethyl-N,N,N-trimethyl chitosan (CMTMC)
synthesis
CMTMC was obtained by O-carboxymethylation of N-trimethyl
chitosan (TMC). TMC was synthesized in two ways, either by
a “conventional” 1-step method (“TMC 1”), or an “optimized”
2-step method (“TMC 2”). CMTMC was further obtained by
carboxymethylation of TMC 2 based either on a “conventional”
protocol previously used in our group (Hansson et al., 2012a)
yielding a product designated “CMTMC 1”, or using a new method