9.5.1.4 Fibrinolytic Activity of Subtilisin and blood-derived proteins (fibrin) are essential for the forma Circulating platelets tion of blood clots, which prevent bleeding long enough for healing to occur. 73 which causes However, excess coagulation prevents normal physiologic means of restoring orders. Thrombolytic therapy is the most direct flow which are blood flow 74 Bacillus spp. produce serine proteases called subtilisi subtilis own to have fibrinolytic activity. 58.75-79 Subtilisin NAT produced by B. admin (natto so called nattokinase) is 9.5% homologous to subtilisin E.38 oral istration of subtilisin induced mi and frequent enhancement of fibrinolyt activit in plasma and production of tissue plasminogen activator Euglobulin activity. degradation products from fibrinTiibrinogen. and the amount of tissue plasminogen activator increased by administration of subtilisin. How whole blood clot lysis time did not significantly decrease. In addition, ingestion of matto decreased euglobulin lysis time and increased euglobulin fibrinolytic activity.m The mechanisms of the fibrinolytic activity of subtilisin NAT are not fully under stood, although subtilisin NAT appears to digest fibrin directly and cleave and inactivate plasminogen activator inhibitor-1 (PAI-1)." Elevation of PAI-1 in plasma is found in patients with thrombotic disease. High PAI-1 activity is related to impaired fibrinolysis, and low activity is associated with bleeding disorders.