A protocol has been developed for in vitro plant regeneration from a nodal explant of
Dracaena sanderiana Sander ex Mast. Nodal explant showed high callus induction potentiality
on MS medium supplemented with 6.78 lM 2,4-dichlorophenoxyacetic acid (2,4-D) followed by
46.5 lM chlorophenoxy acetic acid (CPA). The highest frequency of shoot regeneration (85%)
and number of shoots per explant (5.6) were obtained on medium supplemented with 7.84 lM
N6
-benzylaminopurine (BA). Rooting was high on MS solid compared to liquid medium when
added with 7.38 lM indole-3-butyric acid (IBA). Fifty percent of the roots were also directly rooted
as microcuttings on soil rite, sand and peat mixture (1:1:1). In vitro and ex vitro raised plantlets were
used for acclimatization. More than 90% of the plantlets was successfully acclimatized and estab-
lished in plastic pots. Ex vitro transferred plantlets were normal without any phenotypic aberra-
tions.