Chromosome squashes were prepared from immature
leaves of adult plants used for tissue culture and 3 months
old embryogenic calli. The plant tissues were fixed for at
least 12-24 hours in Carnoy (1 part of acetic acid: 3 parts of
ethanol) followed by hydrolysis in 1 N HCl for 30-60
minutes and then placed on slides and stained with
carbolfuchsin during 30-60 minutes. The tissue was
squashed under a coverslip in a drop of 50 % glycerine