Staphylococcusaureus (S. aureus) is

Staphylococcusaureus (S. aureus) isoneofthemostimportanthumanpathogensandcausesnumerous
illnesses. Inthisstudy,wereportasensitiveandhighlyselectivedual-aptamer-basedsandwichim-
munosensor forthedetectionof S. aureus. Inthisbioassaysystem,abiotinylatedprimaryanti-S.aureus
aptamer wasimmobilizedonstreptavidincoatedmagneticbeads(MB),whichservesasacaptureprobe.
A secondaryanti-S.aureus aptamer wasconjugatedtosilvernanoparticles(Apt-AgNP)thatsensitively
reports thedetectionofthetarget.Inthepresenceoftargetbacterium,anApt/S.aureus/apt-AgNP
sandwich complexisformedontheMBsurfaceandtheelectrochemicalsignalofAgNPsfollowed
through anodicstrippingvoltammetry.Theproposedsandwichassaybenefits fromadvantageousofa
sandwich assayforincreasedspecificity,MBascarriersofaffinity ligandsforsolution-phaserecognition
and fastmagneticseparation,AgNPsforsignalamplification, andanelectrochemicalstrippingvoltam-
metry read-outasasimpleandsensitivedetection.Theelectrochemicalimmunosensorshowsanex-
tended dynamicrangefrom10to1
106 cfu/mL withalowdetectionlimitof1.0cfu/mL(S/N¼3).
Furthermore,thepossibleinterferenceofotheranalogbacteriawasstudied.Toassessthegeneralap-
plicability ofthissensor,weinvestigatedthequantification of S. aureus in realwatersamples.Theresults
werecomparedtotheexperimentalresultsobtainedfromaplatecountingmethod,whichdemonstrated
an acceptableconsistency.

ผลลัพธ์ (ไทย) 1:

Isoneofthemostimportanthumanpathogensandcausesnumerous Staphylococcusaureus (S. หมอเทศข้างลาย)เจ็บป่วย Inthisstudy, wereportasensitiveandhighlyselectivedual-aptamer-basedsandwichim -munosensor forthedetectionof S. หมอเทศข้างลาย Inthisbioassaysystem, abiotinylatedprimaryanti-S.aureusaptamer wasimmobilizedonstreptavidincoatedmagneticbeads (MB), whichservesasacaptureprobeAptamer secondaryanti S.aureus wasconjugatedtosilvernanoparticles(Apt-AgNP) thatsensitivelyรายงาน thedetectionofthetargetInthepresenceoftargetbacterium,anApt/S.aureus/apt-AgNPแซนวิช complexisformedontheMBsurfaceandtheelectrochemicalsignalofAgNPsfollowedโดย anodicstrippingvoltammetryTheproposedsandwichassaybenefits fromadvantageousofaแซนวิ assayforincreasedspecificity, MBascarriersofaffinity ligandsforsolution-phaserecognitionและ fastmagneticseparation, AgNPsforsignalamplification, andanelectrochemicalstrippingvoltam-อ่าน metry-outasasimpleandsensitivedetectionTheelectrochemicalimmunosensorshowsanex-มีแนวโน้มที่ dynamicrangefrom10to1 106 cfu/mL withalowdetectionlimitof1.0cfu/mL(S/N¼3)นอกจากนี้ thepossibleinterferenceofotheranalogbacteriawasstudiedToassessthegeneralap-ofthissensor plicability, weinvestigatedthequantification ของหมอเทศข้างลาย S. ใน realwatersamplesTheresultswerecomparedtotheexperimentalresultsobtainedfromaplatecountingmethod, whichdemonstratedacceptableconsistency

ผลลัพธ์ (ไทย) 2:

Staphylococcusaureus (S. aureus) isoneofthemostimportanthumanpathogensandcausesnumerous
เจ็บป่วย Inthisstudy, wereportasensitiveandhighlyselectivedual-aptamer-basedsandwichim-
munosensor forthedetectionof เชื้อ S. aureus Inthisbioassaysystem, abiotinylatedprimaryanti-S.aureus
aptamer wasimmobilizedonstreptavidincoatedmagneticbeads (MB), whichservesasacaptureprobe.
secondaryanti-S.aureus aptamer wasconjugatedtosilvernanoparticles (Apt-AgNP) thatsensitively
รายงาน thedetectionofthetarget.Inthepresenceoftargetbacterium, anApt / S.aureus / apt-AgNP
แซนวิช complexisformedontheMBsurfaceandtheelectrochemicalsignalofAgNPsfollowed
ผ่าน anodicstrippingvoltammetry.Theproposedsandwichassaybenefits fromadvantageousofa
assayforincreasedspecificity แซนวิช, MBascarriersofaffinity ligandsforsolution-phaserecognition
และ fastmagneticseparation, AgNPsforsignalamplification, andanelectrochemicalstrippingvoltam-
metry อ่าน outasasimpleandsensitivedetection.Theelectrochemicalimmunosensorshowsanex-
มีแนวโน้ม dynamicrangefrom10to1? 106 โคโลนี / มิลลิลิตร ofthissensor, weinvestigatedthequantification ของเชื้อ S. aureus ใน acceptableconsistency

ผลลัพธ์ (ไทย) 3:

staphylococcusaureus ( S . aureus ) ร่วมด้วย isoneofthemostimportanthumanpathogensandcausesnumerous

inthisstudy wereportasensitiveandhighlyselectivedual aptamer , basedsandwichim -
munosensor forthedetectionof S . aureus . inthisbioassaysystem abiotinylatedprimaryanti-s.aureus
, aptamer wasimmobilizedonstreptavidincoatedmagneticbeads ( MB ) , whichservesasacaptureprobe .
เป็น secondaryanti-s.( aptamer wasconjugatedtosilvernanoparticles ( ฉลาด agnp ) thatsensitively
รายงาน thedetectionofthetarget . inthepresenceoftargetbacterium anapt , s.aureus / apt / agnp

แซนวิช complexisformedonthembsurfaceandtheelectrochemicalsignalofagnpsfollowed ผ่าน anodicstrippingvoltammetry . theproposedsandwichassaybenefits fromadvantageousofa
assayforincreasedspecificity แซนวิช ,mbascarriersofaffinity ligandsforsolution phaserecognition และ agnpsforsignalamplification fastmagneticseparation
, ,
andanelectrochemicalstrippingvoltam - กระบวนการอ่าน outasasimpleandsensitivedetection . theelectrochemicalimmunosensorshowsanex -
มีแนวโน้ม dynamicrangefrom10to1
106 cfu / ml withalowdetectionlimitof1.0cfu / ml ( S / N ¼ 3 ) .
นอกจากนี้ thepossibleinterferenceofotheranalogbacteriawasstudied .toassessthegeneralap -
plicability ofthissensor weinvestigatedthequantification ของ , S . aureus ใน realwatersamples ผล

werecomparedtotheexperimentalresultsobtainedfromaplatecountingmethod whichdemonstrated , การ acceptableconsistency .