Estimation of root colonization
At each sample time above, three plants were sampled, and the
roots washed twice in pyrophosphate buffer pH 7. The roots were
then transferred to McCartney bottles containing 10 mL of similar
buffer with 10 glass beads and the yeast cells were detached
by vortexing. The suspension was serial diluted and plated out
on mNA supplemented with 100 g mL−1 tetracycline. The plates
were incubated at 28 ◦C for 24 h before determining the cfu mL