Tomato genotypes King Kong 2 (KK2) and L390, moderately resistant and susceptible to bacterial wilt, respectively, were obtained from the Genetic Resources and Seeds Unit of the Asian Vegetable Research and Development center (AVRDC, Taiwan).
A suspension of a fresh re-isolate of R. solanacearum strain To-udk2 was streaked on NGA agar medium and incubated for 48 h at 28 C.