The
mineral salt media (MSM) with the following composition
(g/L): 0.2 MgSO4, 0.02 CaCl2, 1.0 KH2PO4, 1.0 K2HPO4, 1.0
NH4NO3, and 0.05 FeCl3, and the pH adjusted to 7–7.2 were
amended with 1% filter sterilized crude oil (v/v) according to
Liu et al. [16].