To test whether global optimization of sRAGE coding sequence
will enhance its expression, we transiently transfected plasmids
harboring sRAGE(WT) and sRAGE(OP) to CHO–CD14 cells. Equal
amounts of protein samples from 3 independent transfections
were analyzed with Western blotting, using anti-T7 antibodies.
The expression level from sRAGE(OP) is about 2.6 folds of that of
sRAGE(WT) (Fig. 2), suggesting that optimization indeed impacts
the overall sRAGE autologous expression.