The Black-Pod Rot of cacao (Theobroma cacao) caused by Phytophthora species is one of the most important
diseases affecting this crop worldwide, with average annual losses above 30%. The aim of this work
was to assess the potential for the endophytic Trichoderma martiale strain ALF 247 to control Phytophthora
palmivora. When ALF 247 was applied at concentrations ranging from 1 104 to 5 107 conidia per milliliter,
the disease severity decreased proportionally. Addition of vegetable oil and/or sucrose in the formulations
did not affect the biocontrol results. Fungicides such as copper hydroxide and fosetyl-Al had no
effect on conidial germination of this T. martiale strain, with the germination percentage maintained
above 90%. Once sprayed, the population of T. martiale tended to decrease progressively on the surface
of cacao pods (30–40 days post-application), with a concomitant increase in the severity of pod disease.
Production of 1.02 108 conidia g1 was obtained after culturing ALF 247 on solid substrate (rice grains)
supplemented with calcium carbonate. The results indicate a clear-cut potential of the T. martiale ALF 247
to be used for control of Black-Pod Rot of cacao, although further studies are required to render this isolate
technically and economically efficient as a biocontrol agent on agronomic scale.