Silica-gel TLC-plates (Polygram Sil G) on plastic support were
used. Aluminum support is not recommended because it crucially
hinders soaking in water at the later step. The size of the plates was
20cm×20 cm, 10 cm×20cm or 10cm×10cm (height×width).
The lipid samples (0.1–4L per lane) were loaded without dilution
at a distance of 1.8–2.5cm from each other and 1–2cm from
the bottom. The development was carried out in either 10% ethyl
acetate in hexane (20cm×20cm plates, 1.5 h) or 15% ethyl acetate