The optimized mPCR was performed in 25 mL reaction mixture (PROMEGA; USA) using 1.4 PCR Buffer, 2.5 mM of MgCl2, 0.2 mM of dNTP, 0.2 mM of each primers, 2.5Uof Taq Polymerase and 2 mLofDNA, toppedupuntil 25 mLwith dH2O
The optimized mPCR was performed in 25 mL reaction mixture (PROMEGA; USA)using 1.4 PCR Buffer, 2.5 mM of MgCl2, 0.2 mM of dNTP, 0.2 mM ofeach primers, 2.5Uof Taq Polymerase and 2 mLofDNA, toppedupuntil25 mLwith dH2O