The sheareffect caused by the agitators on the biocatalyst as well as
the operational stability of Lipozyme RM IM was also
taken into consideration. Subsequently, scale-up of the
process to a larger volume (75 L STR) was carried out
using a constant impeller tip speed approach. The optimum
condition of reaction synthesis and physical process
attained from the previous study (Keng et al., 2005) and
from the 2 L STR were used to study the reaction performance
in 75 L STR.