Actinomycetes were identified using oat agar (to observe mycelium formation). For the final distinguishing of the bacteria and for removal of the similar variants we used 16S rDNA restriction analysis, as described earlier (Yakimov et al., 1998), using PCR-based technology. Reagents for PCR amplification were from Fermentas (Lithuania). The forward primer 16F27 (5′-AGAGTTTGATCMTGGCTCAG-3′) and the reverse primer 16R1492 (5′-TACGGYTACCTTGTTACGACTT-3′) were used