Seeds were separated from fruits by using forceps and were sown in various pots and were kept in the shade house of our college to maintain humidity because it is a sun loving plant [7]. The seeds were disinfected with 70% ethanol for 60 minutes followed by three to four rinses with sterile distilled water. This was followed by Surface sterilization with lebolene for 5 minutes and then with systematic fungicide 0.1% bavistin under aseptic condition for 5 minutes, and were washed with sterile distilled water for several times. Then, the seeds were inoculated in sterilized petriplates containing sterile filter paper soaked in doubled distilled water and incubated in the culture room for 7-10 days at 25±2°C. After germination, the seeds with plantlets were transferred to culture tubes containing MS basal medium and allowed to grow for a week [8]. These Plantlets were used for further process. Shoot tip, axillary buds, leaves, nodal and internodal parts of Capsicum chinense were used as the explants for further study.