In conclusion, the results of the current study showed that using 150 g/kg RB in laying hens’ diets adversely influenced the productive performance and eggshell quality, probably due to its potent anti-nutritional factors. Although hens consuming the 0.25 g NPP/kg diet without PHY performed similar to hens fed the diet containing 0.35 g NPP/kg, feeding laying hens with diets containing lower NPP level caused a negative effect on the eggshell quality and stress tolerance. However, including PHY in laying hens diets alleviated the use of high inclusion level of RB or low level of NPP. The responses to added PHY in adequate NPP diets also provide evidence for the impact of supplemental PHY on layers performance independent of its effect on P bioavailability. These findings could be applied for further improvement of commercial laying hens to be reared in tropical climate conditions.