During ebb tide, for each experiment, fifty Plexiglas
circular plates (∅ 7 cm) were placed in an intertidal
mudflat located in the Mondego estuary. To ensure the
attachment of macroalgal spores to the surface of the
Plexiglas plates, because the surface properties greatly
affect settlement success (Lobban and Harrison, 1994), the
plates had a rough surface,which favoured the adhesion of
the spores. The replicates were hung from a rope that
became submerged during high tide. They were placed
10 cm apart from each other and 20–30 cm above the
sediment surface, in order to prevent sediment particles
deposition. Two days later, the plates were collected and
transported to the laboratory in a cooler within 1 h.
During ebb tide, for each experiment, fifty Plexiglascircular plates (∅ 7 cm) were placed in an intertidalmudflat located in the Mondego estuary. To ensure theattachment of macroalgal spores to the surface of thePlexiglas plates, because the surface properties greatlyaffect settlement success (Lobban and Harrison, 1994), theplates had a rough surface,which favoured the adhesion ofthe spores. The replicates were hung from a rope thatbecame submerged during high tide. They were placed10 cm apart from each other and 20–30 cm above thesediment surface, in order to prevent sediment particlesdeposition. Two days later, the plates were collected andtransported to the laboratory in a cooler within 1 h.
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