The suffixes ‘‘pre’’ and ‘‘post’’ indicate the respective values before and after a purification step. To obtain an overall PF and R% for
the 2-step purification approach, we combined the values we
determined for the single purification steps (Table 2). In case one
purification step did not work or could not be evaluated, e.g. due
to too low HRP activity, we only presented the successful purification step. The pooled active fractions after AEC were concentrated
using Amicon Ultra-15 Centrifugal Filter Units with 10 kDa MWCO
(Merck-Millipore, Austria) to the final enzyme preparation of a volume of approximately 1.5 mL.