Lycopene and their cis isomers were quantified at 472 nm and β-carotene was quantified at 450 nm. Since standards of lycopene cis isomers were not available, they were tentatively identified based on retention times and absorption spectrum characteristics described in the literature ( Seybold et al., 2004 and Fröhlich et al., 2007). Carotenoid content was expressed as mg/kg of tomato puree.