2.4. PHA analysisThe biomass concentration was analyzed gravimetrically, the samples were cen-trifuged and the cells were washed with 5% (vol/vol) Triton X (10 ml) and distilledwater, respectively, and afterwards they were dried (105◦C) to the constant weight.The PHB content of dried cells was analyzed by gas chromatography (Trace GC Ultra,Thermo Scientific, USA) as reported by Brandl et al. [23].