Samples were collected from the apical two, fully developedleaves and their chlorophyll a and b (chl a and chl b) contents wermeasured using spectrophotometric method described by Felföldy(1987). According to this method absorbances of the preparedsamples were measured at 653, 666, and 750 nm and chlorophyll a and b contents were calculated using the following equationsafter correction with the value measured at 750 nm:
chl a
=
17.12 A666 −
8.68 A653
chl b
=
32.23 A653 −
14.55 A666
chl a
+
chl b
=
2.57 A666 −
23.6 A653
Pigment contents were then calculated for g per 1 g fresh weight(FW) of the leaves.