In sequential fermentation, prior inoculation of T. delbrueckii played an important role in lowering volatile acids (odour activity value, OAV8 mL/100 mL), higher alcohols (isoamyl alcohol and 2-phenylethyl alcohol) and esters (ethyl esters of octanoate, decanoate and hexanoate, and 2-phenylethyl acetate), relative to the T. delbrueckii monoculture. Therefore, sequential fermentation could accord the wine volatiles from both lychee fruits and biotransformation.