2.2.2. Determination of Bioactive Compounds
z Preparation of Crude Extract: The test method was modified from [24] soaking powders of rice formulas accepted by consumers (before and after cooking) in 95%-methanol solvent to dissolve in distilled water at a ratio of 1:5 (w/v) for 24 hr. The mixture was then spun with a centrifuge machine at 2,500 rpm for 20 min and filtered with Whatman No.1 filtering paper. Next, the mixture was dried under vacuum conditions with a rotary evaporator at 45°C. The rough extracts obtained were used for further experimentation.