The fresh leaves and other parts of healthy plants were collected
and were thoroughly washed with tap water, and were air dried.
Ten grams of plant tissues were ground using pestle and mortar by
adding equal amount (10 ml) of sterilized distilled water (1:1 w/v).
The extract was filtered through muslin cloth. The supernatant was
taken as standard plant extract solution (100%). Further, the extract
was diluted by adding sterilized water to get 10% concentration.