Proximate analysis of the diet and the dorsal muscle
The experimental diets and samples of the dorsal muscle were analyzed for proximate composition based on AOAC (1984) methods. Crude protein was determined with a Kjeltec system 1002 (Tecator). Crude lipid was determined by chloroform-methanol (2:1, v/v) extraction method (Folchet al.1957). Crude fiber was determined by the Fibertec system M 1020 hot extractor (FOSS Tecator). Gross energy was obtained by IKA calorimeter system C 2000 basic. Ash and moisture were determined by conventional methods using muffle furnace at 505°C and an oven at 105°C.