Chlorophyll was extracted from 0.35 cm2 leaf discs in 80% acetone
and quantified as described by Lichtenthaler and Wellburn
. Leaf samples were taken at the end of the day and night periods
and extracted repeatedly with acetone followed by water. Pellets
were boiled for 40 min with 1 ml of 0.1 M sodium acetate–0.1 M
acetic acid and then hydrolyzed overnight at 40 ◦C using 3 units
of -amylase and 0.25 units of amyloglucosidase. Concentration
of glucose was determined using an amplex-red glucose assay kit