All mycoplasmas were grown in their respective required
media (Table 3). ELISA plates were prepared individually
for each organism. Briefly, wells of 96-well plates (Nunc
Immulon 2 HB, Thermo Fisher Scientific Inc., Waltham, MA,
USA) were coated with 100 ml of 10 mg/ml of each antigen
in carbonate-bicarbonate buffer (pH 9.6).