Acid production from carbohydrates
10 ml of medium [NH4H2PO4 (0.5 g), K2HPO4 (0.5 g), MgSO4.7H2O (0.2 g), NaCl (5 g), yeast extract (1 g), agar (12 g) water (1 L), bromocresol purple (1.5% alcohol solution 0.7 ml)] was prepared. A 10% (w/v) aqueous solution of glucose was filter-sterilized through millipore injection and added to the molten base to give a final concentration of 1%. Each isolate was then transferred aseptically into a tube, incubated at 28 °C and checked for acid production from carbohydrates after two, four and six days. Production of yellow color indicated production of acid